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Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.

机译：Hel-N1（一种RNA结合蛋白）的异位表达增加了3T3-L1脂肪细胞中葡萄糖转运蛋白（GLUT1）的表达。

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摘要

3T3-L1 preadipocytes ectopically expressing the mammalian RNA-binding protein Hel-N1 expressed up to 10-fold more glucose transporter (GLUT1) protein and exhibited elevated rates of basal glucose uptake. Hel-N1 is a member of the ELAV-like family of proteins associated with the induction and maintenance of differentiation in various species. ELAV proteins are known to bind in vitro to short stretches of uridylates in the 3' untranslated regions (3'UTRs) of unstable mRNAs encoding growth-regulatory proteins involved in transcription and signal transduction. GLUT1 mRNA also contains a large 3'UTR with a U-rich region that binds specifically to Hel-N1 in vitro. Analysis of the altered GLUT1 expression at the translational and posttranscriptional levels suggested a mechanism involving both mRNA stabilization and accelerated formation of translation initiation complexes. These findings are consistent with the hypothesis that the Hel-N1 family of proteins modulate gene expression at the level of mRNA in the cytoplasm.

机译：异位表达哺乳动物RNA结合蛋白Hel-N1的3T3-L1前脂肪细胞表达的葡萄糖转运蛋白（GLUT1）蛋白最多增加10倍，并且显示出较高的基础葡萄糖摄取率。 Hel-N1是类似于ELAV的蛋白质家族成员，与多种物种的分化诱导和维持有关。已知ELAV蛋白在体外与不稳定mRNA的3'非翻译区（3'UTR）中的短片段尿嘧啶结合，该mRNA编码参与转录和信号转导的生长调节蛋白。 GLUT1 mRNA还包含一个较大的3'UTR，该区域具有一个富含U的区域，该区域在体外与Hel-N1特异性结合。在翻译和转录后水平上对改变的GLUT1表达的分析表明，该机制涉及mRNA稳定和翻译起始复合物加速形成。这些发现与Hel-N1蛋白家族在细胞质中的mRNA水平上调节基因表达的假设相一致。

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Jain, R G; Andrews, L G; McGowan, K M; Pekala, P H; Keene, J D;
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年度 1997
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1. Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. [J] . R G Jain, L G Andrews, K M McGowan, Molecular and Cellular Biology . 1997,第2期

机译：Hel-N1（一种RNA结合蛋白）的异位表达增加了3T3-L1脂肪细胞中葡萄糖转运蛋白（GLUT1）的表达。
2. Tumour necrosis factor-alpha regulates expression of the CCAAT-enhancer-binding proteins (C/EBPs) alpha and beta and determines the occupation of the C/EBP site in the promoter of the insulin-responsive glucose-transporter gene in 3T3-L1 adipocytes. [J] . Jain R, Police S, Phelps K, The Biochemical Journal . 1999,第Pta3期

机译：肿瘤坏死因子-α调节CCAAT-增强子结合蛋白（C / EBPs）α和β的表达，并确定3T3-L1脂肪细胞中胰岛素反应性葡萄糖转运蛋白基因启动子中C / EBP位点的占有率。
3. Prostaglandin F-2 alpha increases glucose transport in 3T3-L1 adipocytes through enhanced GLUT1 expression by a protein kinase C-dependent pathway [J] . Chiou GY., Fong JC. Cellular Signalling . 2004,第4期

机译：前列腺素F-2 alpha通过蛋白激酶C依赖性途径增强的GLUT1表达，从而增加3T3-L1脂肪细胞中的葡萄糖转运
4. Expression of Insulin Signaling Transmitters and Glucose Transporters at the Protein Level in the Rat Testis [C] . KERSTI KOKK, ESKO VERAEJAENKORVA, XIAO-KE WU, Meeting on Cell Signaling World: Signal Transduction Pathways as Therapeutic Targets . 2007

机译：大鼠睾丸中蛋白质水平的胰岛素信号转导和葡萄糖转运蛋白的表达
5. Overexpression of the RNA-binding protein HuD increases the stability of GAP-43mRNA in dentate granule cells of adult mice leading to aberrant synapse formation. [D] . Tanner, Daniel C. 2005

机译：RNA结合蛋白HuD的过表达增加了成年小鼠齿状颗粒细胞中GAP-43mRNA的稳定性，导致异常突触形成。
6. Ectopic expression of Hel-N1 an RNA-binding protein increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. [O] . R G Jain, L G Andrews, K M McGowan, 1997

机译：Hel-N1（一种RNA结合蛋白）的异位表达增加了3T3-L1脂肪细胞中葡萄糖转运蛋白（GLUT1）的表达。
7. Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. [O] . Jain, Renu G., Andrews, Lucy G., McGowan, Kevin M., 1997

机译：Hel-N1（一种RNA结合蛋白）的异位表达增加了3T3-L1脂肪细胞中葡萄糖转运蛋白（GLUT1）的表达。

Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes.

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